Dog boots are commonly used as protective footwear against snow, ice, hot sand, road salt and paw injuries. In veterinary medicine, there are only a few studies that depict the effect of replacement dog boots, such as bandages, on ground reaction forces (GRF) in dogs. To our knowledge, there are no studies that have investigated the effect of dog boots on the center of gravity (COP) of pressure in dogs. This study examined changes in whole-limb GRF and selected paw COP parameters while wearing dog boots in five Labrador retrievers. After habituation, gait and trot data were collected from dogs on a pressure platform without shoes (control measurements) and under five different test conditions (wearing shoes on all limbs, shoes on both thoracic limbs, shoes on both pelvic limbs, one shoe on the left thoracic limb and one shoe on the right pelvic limb). The most significant change was observed when wearing one boot on the left thoracic limb, with a decrease in peak vertical force (PFz%) for the left thoracic limb at trot, resulting in a significant difference between the two thoracic limbs and a significant increase in PFz (%) for the right pelvic limb. In addition, the vertical impulse (IFz%) showed significant differences between the thoracic limbs at both tempos; there was also an increase in the right thoracic limb at trot compared to the control. In addition, some significant changes in COP parameters were noted; for example, all test conditions showed a significant increase in COP area (%) for the right thoracic limb during gait compared to the control. Thus, our results indicate that wearing the tested dog shoes in different constellations seems to have an effect on GRF and some COP parameters.